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  • Recombinant Human TNFα
產品名稱：

Recombinant Human TNFα
產品品牌： SOLARBIO/索萊寶 價格：
廠商性質： 生產廠家 產品時間： 2024-08-01
Recombinant Human Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed.

產品概述

品牌SOLARBIO/索萊寶
Known asTumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member2; TNF-a; TNF; TNFA; TNFSF2
Derived fromE.coli
PurityED50 is less than 0.03 ng/ml. Specific Activity of 3.0 x 10^7 IU/mg.measured by the cytolysis of murine L929 cells in the presence of Actinomycin D.Greater than 95% as determined by reducing SDS-PAGE.
FormulationLyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.0.
ShippingThe product is shipped at ambient temperature.Upon receipt, store it immediay at the temperature listed below.
StorageLyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 13 months
ReconstitutionAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in11X PBS.Please aliquot the reconstituted solution to minimize freeze-t cycles.
EndotoxinLess than 0.1 ng/ug (1 IEU/ug).
Amino Acid SequenceMVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISR IAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
BackgroundTNFα is a homotrimer with a subunit molecular mass of 17 kD cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune diseases and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn’s disease, and rheumatoid arthritis as well as graft-versus-host disease.

Recombinant Human Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed.

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